Sentence examples for selective extension from inspiring English sources
This recommendation was changed in 2009 to 25 years of follow-up after treatment of CIN2-3, aselectivetivextensionof of the screening programme.
Further, despite the competition for the condensing reagent, a sequence-selective extension of the primer occurred in the same mixture, with a rate only modestly slower than those measured in the absence of the other components.
The use of water-soluble organocatalysts (piperidine or pyrrolidinium acetate) to provide the selective chain extension of HMF has been reported recently [46].
Since the cDNA-AFLP procedure uses a two selective nucleotide extension at the 3' terminus of each primer (See methods), there are 256 available primer combinations.
Primers designed by Vos [ 14] complementary to a strand of each of the two adaptors with an additional selective nucleotide extension ("EcoRI-A" and "Mse-C") were used for an initial PCR pre-amplification step.
Finally, 147 independent TDFs were verified by sequence analysis after evaluating the sequence products with expected size, the existence of two selective nucleotide extension, and clone reproducibility (Additional file 1, GenBank accession numbers JK512212- JK512358).
Each PCR was performed at 94°C for 20 sec, 56°C for 30 sec, and 72°C for 2 min. The pre-amplified products were diluted 1 10 and a 4 μl aliquot was selectively amplified in a total volume of 20 μl with MseI and TaqI primers having 1 selective base extension at the 3' end.
Four weeks into her premiership, central to that vision it seems will be an extension to selective education.
"Since the government announced its green paper proposing an extension to selective education, the air has been thick with the sound of claims and counter-claims on the benefits and disadvantages of grammar schools," Carmichael said.
The incremental returns on investment strongly diminish with an extension of 'Selective PCR' to all patients ('Universal PCR'), at an iCER of $232,700 per additional infection averted (line C).
