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On M9 minimal medium, E. coli ΔglmS could grow only when glucosamine was supplemented, therefore, a defined medium could achieve the selective cultivation of the E. coli ΔglmS cells transformed by using glmS as selection marker.
Ancient agriculturists improved many plants through selective cultivation.
Traditional approaches based on selective cultivation or breeding, on the other hand, are more indirect and typically involve modifications of genes and physical features that are already present rather than the addition of fundamentally new characteristics.
coli), blood agar plates (used for selective cultivation of Pasteurella, Staphylococcus and Streptococcus) [50], Marc-145 cell lines for PRRSV [7], etc.
However, in most circumstances, recombinant cells are cultivated in undefined media for fast growing, or are finally applied to nutritional rich environment, and thus an undefined medium has to be developed for the selective cultivation of GM bacteria with GFAT marker.
Stem cell selective cultivation conditions for JLO-1 have been described in our previous study [ 18].
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These methods have become well established through the development of specialized serum free media formulations, selective culture and substrate modifications which allow for successful routine cultivation of keratinocytes [ 13].
Squares were transferred to non-selective co-cultivation media [ 30] for 72 hrs, follwed by MS regeneration/shooting media with kanamycin (300 μg/ml) and cefotaxim (250 μg/ml) until small shoots appeared from the calli.
Increased in seed size is a remarkable result of selective domestication and cultivation [3], [62].
Procedures for the selective isolation and cultivation in monolayer of respiratory cells have been developed.
To resolve this apparent dichotomy, we undertook additional IGS typing directly on serum samples collected from patients with clinical cases, thus removing the selective pressure of cultivation.
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