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This might be caused by strong selective constraints (negative selection) at high temperature [ 19, 20].
This comparison would distinguish between relaxed selective constraints or increased positive selection on male genes.
Positive selection will be taken into account if selective constraints are variable over sites.
Thus, a broad breadth of expression imposes selective constraints limiting the possible action of positive selection.
The selective constraints are characteristic of proteins rather than species.
Selective constraints for each of three chemosensory receptor families were substantially different.
We also estimated the selective constraints for each chemosensory receptor gene family (Fig. 3).
The dependences of selective constraints on amino acid pairs were not taken into account.
Conversely, selective constraints have operated on the expanded PRAMEYs to preserve their essential function in spermatogenesis.
Why the selective constraints acting on selenoproteins have relaxed in Endopterygota remains unknown.
The selective constraints that act on other large exons (B, C and D) are relatively constant.
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