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Background selection was started from BC1F1 to BC3F1 generation and in each step genotype possessing highest genome content of the recipient parent was selected to hybridize for next backcross.
Selection was started 4 hours after nucleofection.
Cells were passaged 24 h post transfections and selection was started 48 h post transfections.
Selection was started 48 hours after infection with 200 µg/ml of hygromycin (Invitrogen) and pools of drug-resistant cells maintained.
The selection was started therefore at 8% O2 and this concentration was gradually decreased by 1% each 3 to 5 generations to keep the selection pressure.
One day after the second infection, puromycin (3 µg/mL) selection was started and GFP expression was monitored by flow cytometry.
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By 2007 the Olympic selection was starting to divide people as Beijing approached.
However, assuming that teleology is acting before the replication process and natural selection are started is equivalent to assume that there is an a priori reason for which there has to be one day, on earth, organisms with eyes, whose goal will be to see.
Jury selection is starting today for the patent trial between Apple and Samsung that has already resulted in the release of tons of early Apple iPhone and iPad prototypes designs.
All antibiotic selections were started at 24 hours after transfections.
A subsample of F1-generation fish from each selection line (N = 50 per selection line) was measured at age 60 days to ensure that there were no initial differences in body size when the selection experiment was started (large-size selected: 15.2 ± 2.79 mm; randomly selected: 15.0 ± 2.26 mm; small-size selected: 15.8 ± 2.58 mm; mean ± SD; χ = 1.8105, P = 0.4044).
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