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Stably transfected clones were identified by screening four weeks after antibiotic selection was removed.
A neoR cassette, which was used for G418/geneticin selection, was removed with Flp recombinase before the ES cells were injected into host blastocysts to generate chimeric mice (Fig. 1C).
Selection was removed 48 h before any experimental analysis.
In the second part of the experiment, thiamphenicol selection was removed to allow growth of double-crossover clones.
First, the rate of loss of plasmids from HeLa and 293 cells from which drug selection was removed was measured directly to be 6.7% per generation (Table II).
After 2 weeks, selection was removed and mass cultures of HeLa and HT1080 cells were passaged at a 1 16 split ratio for 300 pds.
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After a critical amount of time, selection is removed and the episomal vectors are eliminated from the cells during duplication.
All data related to selection is removed.
All loci found to be under directional or balancing selection were removed from the data set.
In a second step, two items which were found to decrease the internal consistency of this item selection were removed.
On the other hand, the capacity to recover the ancestral chromosome when selection is removed endows the evolved strain with increased evolvability in alternative environments.
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