Fixed effects in the model included the cultivation temperature for phenotyping (20° or 30°), the selection regime (ancestor, control-selected, or heat-selected), and the interaction between phenotyping temperature and selection regime.
This generates a legacy of effects that modify and feed back into the selection regime of subsequent generations.
The factors of the model were "environment" (levamisole, ivermectin or control), "generation" (10 or 20), "selection regime" (LE or C lines), a factor "selection line" (C1 C5 and LE1 LE5) nested to the factor "selection regime", and the interactions "environment*selection regime", "generation" * "selection regime", "environment*selection line" and "generation" * "selection line".
Both plasmids were stable under this dual selection regime.
GE: Generation; SR: Selection regime; SL: selection line.
The factors of the model were "generation", "selection regime", a factor "selection line" nested into the factor "selection regime", and the interactions "generation * selection regime" and "generation * selection line".
The significance of Selection regime effect was assessed by comparing the described model with and without the Selection regime effect using chi-square tests.
Replicate line was nested within selection regime and inbreeding level, and replicate cage was nested within replicate line, inbreeding level and selection regime.
A fundamentally different selection regime is in the face of increasing stress.
We calculated mean longevity of the selection regime males for each vial using the mortality data.
This indicates that the selection regime did not affect λ (e.g. via inbreeding).
