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The clones were identified by blue/white selection, processed for plasmid purification with the Wizard Plus kit (Promega), and sequenced (ABI PRISM- 377 DNA Sequencer- Perkin Elmer, Foster City, CA, USA) by using DNA prepared according to the protocol of the sequencing kit DNA Big Dye Terminator (Applied Biosystem- Perkin Elmer, Norwalk, CT, USA).
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