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The implication is that screening of differentially expressed genes can greatly be enhanced by genetic variability and selection of tissue.
Before processing for electron microscopical analysis, MLN sections from control and terminally-scrapie-affected mice were first prepared from a selection of tissue blocks for light microscopy and immunolabelled to detect PrPd, IgG, IgM and ubiquitin.
The criteria for the selection of tissue is described in the supplementary methods.
The selection of tissue blocks taken for microarray and neuropathological analysis depended on the clinical diagnosis of each patient (Table 1).
In order to get a good distribution and random selection of tissue samples throughout the femoral shaft, the sections were made according to five different regions.
We especially thank Jinbo Feng, Haiting Mao Yongmei Yang, and Xiaoying Zhang for excellent technical assistance and advice, and pathologist Qinghui Zhang for selection of tissue and scoring the IHC section.
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The arrays used in this study assay a heterogeneous selection of tissues and biological states.
To complement this approach we included a selection of tissues from shoot and root in order to evaluate Rhizobium-legume interactions and shoot-root communication, which is known to be essential for controlling nodule number [33].
Thus, experimental validation using strand-specific PCR supports the validity of our empirical criteria for the selection of tissue-specific antisense expression.
In a selection of tissues, macrophage identity was confirmed by double staining for PLXND1 and CD68.
Nevertheless, a broad selection of tissues allows for scoring of complex phenotypes affecting any combination of tissues.
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