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Because of the morphological changes of H. pluvialis, microscopic observation was considered during the induction period to facilitate the selection of stress medium.
From the numbers of up- versus downregulated (170 versus 462) transcripts it is easily seen that the bacteria upregulates a selection of stress response genes, while the majority of modulated genes are downregulated to focus on the repair process.
Additional data, with a limited selection of stress types, confirmed strong S0 effects but a lack of S1 and S2 effects for recombination trap lines 651 and IC9 as well (Text S1 and Tables S4, S12, S13, S14, S17).
The selection of stress conditions with contrasting effects on specific metabolic processes helped in understanding their regulation.
The selection of stress agent was a clinical decision based upon using regadenoson by default with patients receiving another form of stress if this was preferred over regadenoson or there was a contraindication to the use of this agent (Fig. 1).
The selection of stress response assays (e.g., apoptosis, antioxidant response, cytotoxicity, DNA damage response, endoplasmic reticulum stress response, heat shock, inflammatory response, mitochondrial damage) is based on the premise that compounds that induce one or more stress response pathway are more likely to exhibit in vivo toxicity than those that do not.
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Our study aims to aid in selection of stress-related TCS members for further functional studies and genetic engineering through a systematic identification of stress-related TCS genes.
To avoid selection of stress-response genes or those normally regulated by NMD, we applied stringent filtering criteria to the NMD microarray data from six breast cancer cell lines.
Investigation of the diversity, biological consequences, and mechanisms of the canonical and noncanonical alternative splicing events will help one to identify those transcripts which are promising for using in genetic engineering and selection of stress-tolerant plants.
To make our database a comprehensive integrated database for functional characterization and selection of stress-responsive TFs, we provided access to tissue-specific expression profiles documented in Genevestigator and RiceXPro through hyperlinks for those TF encoding genes of barley, maize, and rice, for which data are available.
To make our database a comprehensive integrated database for functional characterization and selection of stress-responsive TFs, we have searched public resources for available expression datasets of the six tree crops with the aim to link the TF-encoding genes to their respective expression.
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