It supports our suggestion that during the selection of reference samples, MCV of the selected specimens should be taken into account.
However, how to determine the reference point and whether the selection of reference point will affect the optimality of CVs selected are not answered.
The three selected reference genes were analyzed in the complete dataset, and the final selection of reference gene/s was established using the complete dataset by means of both low sample-to-sample variation and an absence of group differences in expression.
Hence, the selection of reference AP is of vital importance.
Additionally, these results provide useful guidelines for the selection of reference genes in other Alternaria species.
Interestingly, the UCQY values depend on the selection of reference material and concentration of NPs.
The results of this study provide useful guidelines for the selection of reference genes in other Alternaria species.
The tolerance stack-up and selection of reference surfaces for the subsequent machining operations is inferred from the diagram.
A critical step in an RT-PCR study is the selection of reference genes for data normalization.
where ϕ n is the AoD, the minimization over Δϕ is to eliminate the additional angle spread introduced by different selection of reference zero angle.
For the results to be dependable, selection of reference genes that express consistently across the tissue, genotype and developmental stage variables is critical (Wong and Medrano 2005; Guénin et al. 2009).
