Sentence examples for selection of expressing from inspiring English sources

Selection of expressing plants was performed either using a confocal laser scanning microscope (CLSM; see below) for plants containing GFP, or by staining for GUS activity (see below).

The ease with which close-to-optimal expression is obtainable in a single step with the new approach, coupled with the target protein-independent selection of expressing cells, offsets some of the risk in producing hard-to-assay proteins, as exemplified by our expression of biotinylatable CCL18.

Our findings show that chondrocytes, regardless of pathology, exhibit extensive control over the turnover of a selection of expressed transcripts.

These vectors contain four different internal ribosome entry site-selectable markers, allowing high-efficiency selection of transductants expressing the desired cDNA.

After initial selection of cells expressing GFP or GFP-EGFR, protein expression and cellular localization were analysed by Western blot and by fluorescence microscopy, respectively.

This may be due to the selection of highly expressing cells for patch-clamp, the over-expression of target protein in the dual-fluorescence assay and the background level of Kir2.1 protein from untransfected cells.

The selection of highly expressing clones and the optimization of certain culture parameters resulted in protein expression levels of 100 170 mg/l.

ten Botekel, E., Yamagami, T., Andersson J., Rolink, A. G. & Melchers, F. The formation and selection of cells expressing preB cell receptors and B cell receptors.

Cell culture supernatants were screened by enzyme-linked immunosorbent assay and Western blot for selection of high expressing clones followed by sequential protein purification with anion exchange and hydroxyapatite columns by fast performance liquid chromatography (FPLC) [5].

The ClonePix 2 System offers the necessary sensitivity to detect endogenous levels of protein limited to cell surface expression by utilizing robust white light and fluorescent imaging, followed by rapid selection of high expressing clones.

The selection of clones expressing GHR-Rluc was done using Geneticin (Invitrogen).