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Lipofection transfection of E. histolytica trophozoites with the constructed plasmids, drug selection, maintenance of selected transformants, and indirect immunofluorescence analysis of independent established transformants were performed as described previously (5).
For selection maintenance, the cells were selectively grown in a medium containing 100 μg ml−1 geneticin.
The recommendations related to work organisational change (i.e. work scheduling and work practises such as teamwork and job extension, communication/social support networks) and organisational strategies (i.e. reporting systems for musculoskeletal ill heath; procedures for risk assessment; training programme design; equipment selection; maintenance procedures; workplace changes).
AAS, HDCF and MAM were responsible for helping in conceiving the study, as well as Xf strain selection, maintenance and DNA extraction.
Our findings have important implications for the selection, maintenance, and treatment of drug-resistant members of the Enterobacteriacea, predicting that fluoroquinolone-resistant strains may be naturally maintained even if the use of this crucial group of antimicrobials is restricted.
Through optimization by natural selection, maintenance effort is believed to settle below the level that is required to prevent aging (Kirkwood 1977; Kirkwood and Holliday 1979; Kirkwood and Rose 1991; Drenos and Kirkwood 2005).
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Also, three categories of selection of maintenance strategy, maintenance support organization and scheduling of maintenance tasks are the most addressed areas of research.
Consistent with the role of reward motivation as a critical determinant of goal selection and maintenance, motivational manipulations (i.e., reward incentives) have been found to enhance cognitive maintenance and proactive control.
Stable transfectants, inducibly expressing EGFP-scNS3 or EGFP-Full NS3-4A were selected in a medium containing zeocin (100µg/ml and 50µg/ml for selection and maintenance, respectively) (CAYLA, France).
Stable transfectants, constitutively expressing EGFP-Full NS3-4A were selected and maintained in a medium containing zeocin (100µg/ml and 5µg/ml for selection and maintenance, respectively).
Chapter 7 deals with selection and maintenance.
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