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To isolate naïve CD4+ T cells, CD4+CD25− T cells were stained with biotinylated anti-CD45RO mAbiotinylated anti-CD45ROls were depleted using Easy-sep biotin selection kits; simAbandy thensolate resting memory CD45RO+cells, CD4+CD25− T cells were stainedepletediotinylated–anti-CD45RA mAb and CD45RA+ T cells were depleted using Easy-sep biotin selection kits.
This has led to the marketing of negative selection kits yielding "untouched" cells.
Mature monocytes or granulocytes from six healthy volunteers were isolated from peripheral blood using the EasySep selection kits nos.
Human NK cells were isolated from normal control PBMCs using positive or negative selection kits (Miltenyi Biotech, Auburn, CA).
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Lymphocytes were selected from PBMC using an immunomagnetic-based selection kit from Miltenyi Bioytec (Auburn, CA).
Control genes were selected using the geNorm™ human Housekeeping Gene Selection Kit (Primer Design Limited).
(One birth-planning center offers a "baby gender selection kit" involving three safe and natural steps that turn sex itself into a gender-reveal party).
At the end of the cell induction, all the cells expressing CD11c in the different wells were isolated respectively using the Mouse CD11c Positive Selection Kit (EasySep®Magnet, StemCell Technologies, Vancouver, Canada) according to the manufacturer's instruction and seeded into new wells with fresh medium.
CD4 T cells were MACS purified using a negative selection kit from Miltenyi.
Lineage+ cells were depleted prior to sorting using the EasySep Cell Biotin Selection Kit (StemCell Technologies Inc).
They were sorted magnetically using the positive selection kit purchased from Miltenyi (Bergisch-Gladbach, FRG).
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