Some of the systems didn't work at all; they displayed incorrect selections, froze up, acted generally odd.
After mating and replica-plating (see before), we subcultured F1 hybrids from solid medium into liquid YPD (96-well plate filled with 150 μl medium/well) using the ROTOR, incubated the cells overnight (horizontal shaker 900 rpm, 30°C), subcultured them the next day into fresh YPD + 5% (v/ v) ethanol (selection), and froze the cells down (no selection).
It is also possible that could lead to a population size that is large enough to cause extreme purifying selection, essentially freezing ribosomal sequences.
No morphologically differentiated sex chromosomes were ever found in species belonging to the non-Antarctic notothenioid families (Bovichtidae, Pseudaphritidae, and Eleginopidae) [ 36, 37], distributed in the sub-Antarctic region of the Southern Ocean, where they had not been exposed to natural selection by freezing marine conditions in their evolutionary history [ 17].
The selection has been frozen in the legislature, which was already up in arms over the president's contentious choice for attorney general: Raúl Cervantes, a close ally of Mr. Peña Nieto and the main lawyer for his party during the 2012 campaign.
The red checkered tablecloth, the fruits in the fruit bowl, the colored coffee mugs with their scratched off insignias, and the cabinet's best selection of boxed cereals, frozen momentarily in arbitrary formations, all illuminated by morning light.
We've frozen the mentor selection for now until the we've selected the class before we select the final 10 or twelve mentors to ensure they're really tailored towards those companies".
ES cell selection, colony picking, freezing, expansions, and cryopreservation of homologous recombinant clones expressing the R120G mutant αB-crystallin gene were performed at the Washington University ES Cell Core facility.
For initial selection and categorization, freezing was assessed by separating the monkey from its cage mate and relocating it to a test cage in which for 10 minutes it was exposed to the potential threat of a human intruder presenting her facial profile to the monkey [no eye contact condition (NEC) of the human intruder paradigm] (Fig. 1a).
Colonies from the final haploid selection plates were pooled and frozen at −80° before testing.
