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Guidance is given on the design, construction and operating criteria of the process units including typical design criteria and characteristics, filter configuration, media selection, bed depth, underdrain systems and the operation of the units; filtration and backwash rates, flow control, headloss, air binding, negative head and solids retention.
The selection bed was then rinsed with 1 mL of PBS (pH = 7.4).
Two 150 μm wide inlet and outlet channels poised orthogonally to the isolation channels addressed the selection bed.
Stable attachment of mAbs to the surfaces of the selection bed, which were made from a polymer, is critical for efficient recovery and purity of the target cells.
CD4+ T-cells and neutrophils isolated on-chip were lysed by infusing ∼20 μL of lysis solution (GenElute Mammalian Total RNA Miniprep Kit, Sigma, St . Louis MO) through the cell selection bed.
Similar(55)
The architectures for the 2 devices evaluated herein differed in terms of addressing the selection beds (4 per device), employing either a bifurcation network or Z-configuration.
While the bifurcation design could be configured with a larger number of channels to increase throughput, it comes at the expense of device footprint; the 64-channel Z-configuration occupied an area of 35 × 45 mm (4 selection beds) while the same number of channels for 4 selection beds using the bifurcation configuration would have required an area of 128 × 160 mm.
The microfluidic device consisted of four independent selection beds with curvilinear channels that were 25 μm wide and 80 μm deep and were modified with antibodies targeting antigens specifically expressed by two different cell types.
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