Exact(21)
Bacteria from fresh selective agar plates were inoculated into 2 ml LB medium with selection antibiotics and incubated over-night at 26°C with shaking.
Many ocular cells including RGC5 are very resistant to various commonly used selection antibiotics, including G418, zeocin, and puromycin.
This two-step Tet-on system is not only time consuming but also reliant on the cell's sensitivity to at least two different selection antibiotics.
Depending on the resistance genes present, medium was supplemented with additional selection antibiotics G418, Hygromycin (Roche Diagnostics), Ouabain (Sigma), or Blasticidin (InvivoGen).
After a 3-day-incubation with lentivirus, cells were trypsinized, transferred to 10-cm2 dishes and allowed to grow in presence of the selection antibiotics, puromycin (Sigma, St-Louis, MO).
For experiments, the cells were passed in a medium lacking selection antibiotics 2 4 days.
Similar(39)
Following transduction, puromycin (1.5 μg ml−1) was used as a selection antibiotic to select the infected cells for 10 days (Li et al, 2013).
After an appropriate number of days/passes stable lines were selected and cultured in the presence of the selection antibiotic.
After an appropriate number of days/passes, several stable lines were selected and cultured in the presence of the selection antibiotic (600 µg/ml geneticin or 300 µg/ml hygromycin).
Geneticin selection antibiotic (Invitrogen) (600 μg ml−1) was used to select transfected cells and eventually create stable transfectants.
Two days after infection, medium containing the appropriate selection antibiotic was added and stably transduced cell lines were selected.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com