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Mutant seeds were germinated and selected on selection agar plates [1/2 MS, 1% sucrose (w/v), 25 μg/ml hygromycin, 0.8% agar (w/v)].
Bait and prey plasmids were co-transformed into the Saccharomyces cerevisiae L40 reporter strain and grown on selection agar plates lacking leucine and tryptophan to select for plasmids.
To prevent MRSA being overlooked, some selection agar media have recently been developed.
The colony surfaces of yeast on each selection agar plate are uniform.
We evaluated six commercially available selection agar media in regard to the detection of 35 borderline MRSA (BOMRSA) strains which were mecA-positive but showed low resistance to oxacillin.
TSA and LB sucrose selection agar presented a satisfactory selection.
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Several E. ictaluri ΔasdA mutants were recovered from TSA and LB sucrose-selection agar plates.
Since colonies with a diameter of <1.5 mm did not grow well in the freezing media, only those colonies with a diameter of >1.5 mm on selection-agar plates were picked.
Second, we chose to perform selections on agar plates to emphasize selection for survival instead of growth rate at increased aminoglycoside concentrations.
For selection LB agar plates with 34 µg/mL chloramphenicol was used.
The fragments were ligated into pSK5630 [75] following digestion with BamHI/SalI, and the resulting constructs and the control plasmid were transformed into E. coli DH5α, with selection on agar plates containing ampicillin.
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