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Cultures were carefully selected under the microscope to ensure that only plates showing uniformly growing cells were used.
The tumor sections were first selected under the microscope to ensure that they comprised at least 70% neoplastic cells.
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Selection for fluorescent transgenic plants was done by the addition of 2 µM MSX to the plates and the most strongly expressing plants were selected under the fluorescent microscope.
For the infected progeny of PD8753 parents, 16 dcr-1 and 16 dcr-1/+ adults per replicate were selected under the fluorescent microscope (i.e., the balancer chromosome carries a gfp reporter) and lysed individually in 10 µl of lysis solution (with 1 100 DNase I) from the Power SYBR Green Cells-to-Ct kit (Ambion).
Good quality COCs, judged morphologically with multiple cumulus layers and homogenous cytoplasm, were selected under the stereo microscope and washed repeatedly in modified phosphate-buffered saline (PBS, supplemented with 36 mg mL-1 pyruvate, 50 mg mL-1 gentamycin, and 0.5 mg mL-1 bovine serum albumin fraction V).
The number of multinucleated cells in five randomly selected areas under the microscope was counted.
Thus, grains with a diameter comparable to that of a human hair, selected under a microscope to be crack-free and of the highest possible quality, have been found to be more concordant than cracked grains.
Whiteflies were anesthetized using CO2 and four days old females were selected under a binocular microscope by the morphology of the abdomen.
Venules (30 40 μm) were selected under a microscope.
Five vision fields were randomly selected under a BX50 microscope (Olympus, Tokyo, Japan) and the number of cells that penetrated the membrane was counted.
The positive plaque was selected under a fluorescence microscope and subsequently confirmed by an immunohistochemical assay using an S glycoprotein specific antibody (Fig. 1B, left image).
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