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Three fields were randomly selected under a light microscope (200× magnifications) for cell counting and photography.
Three areas of most intense vascularisation were selected under a light microscope with a 100-fold magnification.
Resin-embedded blocks were cut into sections, 1 μm in thickness, stained with toluidine blue, and then the areas of interest were selected under a light microscope.
To quantify the number of ALP-positive cells and the total number of cells and calculate the positive staining rate, 10 non-overlapping fields of view were randomly selected under a light microscope.
The athymic nude rat is T-cell deficient, but has normal complement and B-cell function [ 11]. 10 tumour spheroids (250-350 μm in diameter) were selected under a light microscope.
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Transformants expressing GFP were selected under ultraviolet light with a Zeiss Axio imager A1 microscope.
Digital images of apoptotic cells were selected randomly under a light microscope.
The percentage of motile spermatozoa was determined by counting more than 200 spermatozoa in randomly selected fields under a light microscope.
Quantitative analysis of BrdU-positive cells was performed by counting the positive nuclei from ten randomly selected fields under a light microscope at ×400 magnification.
The number of cells that migrated to the lower side was counted in five randomly selected fields under a light microscope.
These embryos were selected under normal light illumination to prevent biased selection under GFP fluorescence.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com