The selected transformations indicate that the proportion of sailfish declines rapidly at first with increasing distance, and then to a lesser extent.
It has been reported that g fp expression in transformed cells is useful to select transformation events in early stages, so that antibiotic selection is not needed [ 47, 54- 61].
The gfp expression in transformed cells should be useful to select transformation events at early stages, so that antibiotic selection marker genes may not be required.
An advantage of visualizing GFP expression in our system was to enable us to select transformation events at an early stage thus avoiding the transfer of non-transgenic shoot that survived the kanamycin selection, saving both time and labor.
While transformants were found after transformation with DNA and PCR products from the BZ586 claR strain, none of the transformants were selected after transformation with DNA from the claS strain 26695 or TE.
Agrobacteria selected for transformation were used to transform Col-O plants using a floral dipping method as described [39].
Several transformations were tried for each nonnormal data set before further analysis, and the best transformation for the each data set was selected (the transformation that produced the best fit to normality and homogeneity of variance).
