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Six up-regulated and 4 down-regulated circRNAs from the top 10 candidates were eventually selected to be validated.
Let-7c and miR-589 were selected to be validated in CA group and miR-425* and let-7d* were selected to be validated in AA group.
In this way, genes functionally related to muscle and fat cell metabolism were selected to be validated by qPCR in a subset of 10 animals from each group.
Fourth, some of the predicted direct target-relationship miRNA-mRNA pairs were selected to be validated in an extended cohort of CRC tissues by qRT-PCR detection.
In the study of Ramayo-Caldas et al. [ 10], the CNVRs selected to be validated were detected by at least two programs and were of high frequency, whereas CNVRs selected to be validated herein were detected by one program, with low to high frequencies.
A subset of 13 proteins (highlighted in yellow on figure 3) found to be differentially abundant between CD and HC on the basis of 2D-DIGE were selected to be validated using a targeted LC-SRM assay.
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We selected genes to be validated by quantitative RT-PCR (qRT-PCR) in some specific functional classes: development, differentiation and proliferation, signal transduction and trafficking, cell defense and finally gene transcription and modification.
We ascribe this small fraction to our selection criteria implemented in selecting TUs to be validated.
Nevertheless, we selected five SNPs to be validated in a combined dataset of two independent studies (Table S2 in Additional file 3).
The expression of 18 genes (two transcripts of hmbsb were analyzed separately) was selected to be detected by qPCR to validate the RNA-seq data.
The expression of 15 genes from cluster III, IV and VII was selected to be measured by qPCR to validate the RNA-seq data.
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