The lengths of each selected shoot were measured at about weekly intervals throughout the growing season.
Photosynthesis was measured in situ in all leaves along selected shoots over the growing season.
Approximately 1 month later (mid-March), the selected shoots on each vine were destructively harvested and taken to the laboratory.
All leaves on each of two of the selected shoots in each panel were measured at about weekly intervals.
Measurements of the lengths of each bunch on each of the six selected shoots on each vine were also measured at regular intervals after flowering had occurred.
All leaves on the selected shoots were measured on each occasion; thus an increasing number of measurements was required because the shoots developed up to 30 leaves as the season progressed.
For each treatment, a minimum of 30 shoots were used, and leaves were sampled from three randomly selected shoots at each time point and mixed as a material pool.
All bunches on the selected shoots of each vine were harvested by about 12 February, taken to the laboratory and dried at 60 °C for 2 weeks to determine dry matter content.
Gas exchange parameters were determined on the middle leaflet on the third most recently emerged leaf on two randomly selected shoots using a portable infrared gas analysis system (LI6400XT; LI-COR Biosciences, Lincoln, NE, USA).
