We have shown previously in a selected set of mutant proteins that chemical chaperones may restore biological function of CBS mutants [ Singh et al., 2007].
From these experiments we could confirm the observed positive epistatic effects in a selected set of double mutants from our screen of heterozygous combinations in a reciprocal direction and/or homozygous status.
To determine whether Binding SPLN or the Composite SPLN was enriched for short- or long-lived deletion strains relative to the randomly selected set of 564 deletion mutants [14] or the current data set for the entire deletion collection, a Fisher's exact test was performed using the Fisher's exact test calculator at http://www.langsrud.com/fisher.htm.htm
Briefly, we made P22 lysates from a selected set of Tn 10 insertion mutants of S. typhimurium LT2 by growing a 3 ml overnight culture in LB broth of these selected Tn 10 mutants and inoculating these cultures with phage P22 at a multiplicity of infection of 1 100, followed by co-incubation for 6 hours.
The GO Term Finder function (Boyle et al., 2004) using the default settings which includes noncoding genes was used to calculate P values describing enrichment of GO terms in the selected set of hygromycin B-sensitive mutants compared to the 7167 gene elements in the yeast genome.
To understand the contribution of the individual D6PK genes to auxin transport in inflorescence stems, we measured basipetal auxin transport in primary inflorescence stems of a selected set of d6pk single, double and triple mutants that represented a previously established phenotypic series (Zourelidou et al., 2009; Willige et al., 2013).
The constraints imposed by gene expression levels of mutants can be used to address a selected set of questions about a gene network.
We compared the time-course expression profiles between FHB-resistant Wangshuibai plants and susceptible Meh0106 mutant plants of a selected set of genes that are critical to the plants' resistance and defense reactions.
However, analysis of a selected set of genes showed that the effect of novobiocin observed in the arl mutant strain is similar to that in the wild type, indicating that novobiocin affects gene transcription independent of arlRS.
In TAF4-mutant liver, 1408 genes were down-regulated and 776 up-regulated compared to wild-type The changes in expression of a selected set of genes were confirmed by RT-qPCR.
