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At least five randomly selected serial sections were examined per pancreas and three mice were analyzed for each diet/genotype group.
Selected serial sections were subjected to immunohistochemistry with the Histostain-Plus Kit LAB-SA Detection System (Invitrogen, USA) according to the manufacturer's protocol.
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If you need to delete serial ports, select virtual serial ports pair, switch to "Manage ports" and than "Delete pair".
Of the 30 nucleotide substitutions selected during serial passage, 53% resulted in amino acid substitutions.
Mutants were selected by serial transfers in AM1 mineral salts medium with 10% xylose.
The emitter radionuclides were selected in serial calculations; and the best "candidates" have been identified for application in this procedure based on a set of characteristics.
For each tumour, 10 randomly selected non serial sections were studied.
Only viable tumor cells from disaggregated H1299 xenografts were selected for serial tumor transplantation into secondary, and subsequently, tertiary recipient mice.
In the first step, an intermediate virus, harbouring the fully integrated recombinant plasmid and both the wild type and deleted versions of the gene, is selected by serial passage in the presence of puromycin.
As for the TMA, a significant number of normal and tumor samples showing nuclear staining were randomly selected and serial sections probed with the ENO-276/3 ENO-276/3s specific for full-length α-enolantibodiesigure 2C for represpecifice immunohistochemistry).
Tumor cells were selected by serial transplant into the cleared mammary fat pads of SCID/Beige immunocompromised mice and the resulting tumor tissue passaged in monolayer and 3D culture for the experiments described herein.
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CEO of Professional Science Editing for Scientists @ prosciediting.com