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It's a busy time – two new books are about to be published; a children's poetry book, I am a Poetato, and New and Selected Potatoes, a collection of new works and past favourites.
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Next, a construct containing both cisgenic late blight R genes (Rpi-vnt1.1 and Rpi-sto1), but lacking the bacterial kanamycin resistance selection marker (NPTII) was transformed to the three selected potato varieties using Agrobacterium-mediated transformation.
Recently, FISH has been used to survey CNVs using 18 randomly selected potato (Solanum tuberosum) BAC clones in 16 potato cultivars with diverse genetic backgrounds.
The selected potato cultivars are categorized as maincrop ('Russet Burbank') and early maincrop ('Sylvana') according to the British Potato Variety Database (2009).
Each sample tissue was a collection of two randomly selected potato tubers harvested from the same transgenic line or wild type.
While GC content and exon/intron length were similar between syntenic tomato/potato BACs and randomly selected potato BACs, the average gene in randomly selected BACs had one less exon per gene and consequently were shorter (2.4 kb vs 3.1 kb).
The lysine insensitive AK was used by Perl et al. [ 25] as a selection marker to select potato.
We developed a marker-free transformation pipeline to select potato plants functionally expressing a stack of late blight R genes.
Select potatoes of a similar size.
The transformants were selected on potato dextrose agar (PDA) (ATCC medium 336) supplemented with 50 μg/mL hygromycin and purified to uninuclear clone by diluting spores on PDA plates.
We selected two potato BAC clones which contain telomeric repeats on both end sequences and six BAC clones in which only one of the ends contained the telomeric-repetitive sequences (Additional Data File 5).
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