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The Bayesian random-effects method detected ten positively selected positions with very high confidence (posterior probability >95%) and another four with less confidence (posterior probability 90 95%) (Table 4).
Mutation experiments of D1 protein will unveil the functional importance of the positively selected positions.
The positively selected positions were compared to the human Swiss-Prot sequence (Q15831).
However, ancestral sequences of PpDAM genes did not shared the same nucleotides (probability higher than 0.90) in the non-positive selected positions 238 and 240 around this site.
Both models identified 4 codons (codons number 7, 9 25 and 27) under strong positive selection, and a number of less strongly selected positions (Figure 3).
If a test is significant, Bayesian prediction is used to identify the selected positions or lineages affected by selection.
After averaging of the predicted ΔΔ G values for the twelve selected positions, we made the final selection of the four candidate mutations.
MALDI-TOF MS revealed cleavage of the polypeptide backbone of Mb at selected positions.
The frequency analysis of examined phenomenon is done by the fast Fourier transformation (FFT) at selected positions in the images.
Monosaccharides provide an excellent platform to tailor molecular diversity by appending desired substituents at selected positions around the sugar scaffold.
Among genomic position selected, positions with + TAP signal lower than noise cutoff (50th percentile of signals from all genomic positions) were discarded.
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