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This last approach is particularly well-suited to our application, where whole SNP data from divergently selected lines are available.
Data obtained from clonally selected lines are potentially compromised by clonal bias 6, 26, an issue avoided by lentiviral transduction 1.
The resulting measurements of leaf length, width, and plant height for selected lines are presented in Additional file 2: Figure S2.
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For individual sampling, concentrations at selected single stations and stations along selected lines were monitored by one person at different times.
After phenotyping these hybrids in the laboratory, selected lines were tested in the field.
FL478-derived Saltol NILs were developed as follows: 140 FlinesIR29 BC3F4 lines were genotyped with 28 SSR markers and 55 selected lines were screened for tolerance in the phytotron.
However, the DTF of the selected lines were in general lower than that of MRQ74, indicating that the MRQ74 PLs flowered earlier than the recipient parent.
The carotenoid composition of tubers and leaves from selected lines was analyzed via diode array HPLC.
Therefore it seems very likely that differences in Glo1 expression in the selected lines were due to the duplication; a combination of genetic drift and inbreeding might have caused this apparently surprising result.
All selected lines were demonstrated to be pluripotent, and form teratomas in vivo (Fig 1D).
The selected lines were advanced to preliminary yield trials in the third summer after the cross.
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