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Transformants were selected for uracil protrophy.
Transformants were selected for uracil prototrophy by plating on synthetic media lacking uracil (SC-Ura−).
Transformants were selected for uracil prototrophy.
These constructs were introduced in yeast and transformants were selected for uracil prototrophy.
Plasmid transformants were selected for uracil prototrophy, resulting in plasmid integration at the celA locus.
S. cerevisiae was transformed with the lithium acetate dimethylsulfoxide method [ 48] and selected for uracil prototrophy on SCD-URA.
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Transformants were plated onto SD agar containing histidine, adenine, and leucine to select for uracil prototrophy.
Transformants were plated onto SD agar with histidine, lysine, and methionine to select for uracil and leucine prototrophs.
Selecting for uracil auxotrophy and G418 resistance identified colonies exclusively dependent on the plasmid encoding mutant KRS1.
Expression plasmids carrying the human CBS major allele, or variants, were transformed into this strain by selecting for uracil prototrophy (Ito et al. 1983).
This medium selects for both uracil and leucine prototrophy, which are linked to h and the forespore membrane marker GFP- psy1 +, respectively, and for the SPB marker sid4 +-tdTomato:: hphMX6.
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