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According to the simulations, the power switching control tables are searched and selected for successful switching.
Cells were infected with shRNA-expressing lentivirus, briefly selected for successful proviral integration.
Sec13-depleted blastomeres were transplanted into wild-type albino embryos and selected for successful transplantation at 1 dpf.
Cells were then selected for successful transfection by GV118-GFP-shRNA-IKKα lentiviral vectors and treated with the homologous lymphotoxin-LIGHT (R&D Systems).
Key parameters such as the plating solution matrix, plating flow rate, analysis flow rate, solution composition and square-wave parameters have been characterised and optimal conditions selected for successful and rapid analysis of Co II) and Ni II) at the ppb level.
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Integrating a tetracycline resistance cassette in the first step enabled us to select for successful recombinants after the second recombination using growth suppression of Tetr clones on Bochner-Maloy plates [9], [10].
Transformants were selected on 100 mg/liter spectinomycin to select for successful incorporation of the minitransposon which contains a spectinomycin resistance gene, aad9.
The cells were resuspended in a small volume and plated on SD medium without Leu and Trp to select for successful transformation.
Strains were crossed to MAT a or MAT α tester strains and replica-plated to media that selects for successful mating.
Transfected cells were plated at single-cell density and cultured with Puromycin (1.5 µg/ml) to select for successful recombinants and with Tetracycline (1.0 µg/ml) to repress transgene expression.
However, with no Harlequins players selected for the successful summer British & Irish Lions tour to Australia, you feel their senior players will have a point to prove.
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