Exact(5)
UV1 and UV2 showed maximum zone of clearance on CMC agar plate with a cellulolytic index of 34 and 36 mm (data not shown) and were selected for strain improvement studies (Additional file 1: Table S1).
In addition, pre- and post-treatment isolates were selected for strain typing from all cows receiving lactation therapy.
Samples are selected for strain characterization on the basis of representativeness in terms of seasonal and age distribution and their quality.
In quarters having more than one isolation event over time, isolates were selected for strain typing from early (e.g. the first isolation event), middle (one or more subsequent isolation events), and late (one of the final isolation events) time points.
Presence of genetic markers associated with penicillin resistance (blaZ) and methicillin resistance (mecA) were tested by PCR using established methods [ 26, 27] for all isolates selected for strain typing, and these isolates were also screened for beta-lactamase expression using a nitrocefin assay [ 28].
Similar(55)
Alternatively, we can also speculate that adaptation in quail and chickens has selected for strains with improved replication efficiencies.
This might, as suggested (10 ), have selected for strains that are more efficiently transmitted by adolescents and adults, in whom immunity has waned.
In this process, the strain was kept under constant xylose limitation in a chemostat, and the resulting pressure selected for strains that are best able to use xylose as a carbon source.
These isolates have been stored and subcultured since the time of their isolation from patients or the environment, and this prolonged period of in vitro growth in complete medium may have selected for strains with a haploid complement of chromosomes.
Since we did not find multiple strains in original fluids we were unable to determine whether culture may select for strains that grow more efficiently.
In addition, it was possible to compare strains sequenced directly from body fluids to those sequenced after cell culture isolation, to address whether growth in culture might select for strains that grow more efficiently and therefore mask the detection of multiple strains.
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