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The MS/2 salt concentration that enabled efficient embryogenic callus proliferation and subsequently efficient transformation was thus selected for further transformation experiments.
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Four of them (GRSW1-B1, GRSW2-B1, CPSW1-B1 and CPSW2-B1) exhibited relatively good tolerance at 1%v/v, but due to the limitation of genetic transformation feasibility (as described later), isolate GRSW2-B1 waselecteded for further investigation.
Five independent transformation experiments were conducted and 4 independent transgenic lines were selected for further analysis.
Two data transformation methods were utilised with only markers that were significant in both selected for further analysis.
After transformation, plants that are homozygous and contain one copy of the transgene are typically selected for further study.
Two transformation events targeting BdCAD1 (amiR-cad1-1 and amiR-cad1-8) and three transformation events targeting BdCOMT4 (amiR-comt4-3, amiR-comt4-5, and amiR-comt4-7) were selected for further characterization in the T2 generation.
ZnO was subsequently selected for further studies as support for Rh, Pt, Pd and Au and the resulting solids were tested again for glycerol catalytic transformation under reductive or inert atmosphere at 453 K.
The darkest spotted culture was selected for further characterisation.
It is selected for further improvements.
Four flash flood events were also selected for further analysis.
These derivatives will be selected for further biological investigations.
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