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Enzymes selected for activity measurements were prepared as N-terminal His-tag fusion proteins, while those for crystallization were produced without a His-tag.
To obtain such rich information, the shortest possible epoch (1 s) should be selected for activity monitor data collection [95], primarily to maximise the original PA related bio-signal being retained.
The regions selected for activity concentration did not include tumors, and we make the assumption that scaling these regions by whole-organ mass appropriately reflects normal tissue uptake of FDG.
A number of structurally similar compounds were arbitrarily selected for activity studies, mostly based on price and commercial availability.
We exclusively selected for activity enhancing mutations because we consider them to be much more informative as to chaperone mechanism than loss of function mutants.
RNA-cleaving DNAzymes have been selected for activity in several different metal ions, including Mg2+, Ca2+, Zn2+, Mn2+, Pb2+, and UO22+ (reviewed in ref (166)).
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According to PMI standard (1996) triangular distribution is selected for activities and costs.
Also, the following assumptions were considered in constructing the model: According to PMI standard (1996) triangular distribution is selected for activities and costs.
We have clarified our rational for looking at activity enhancing mutations by adding these sentences to the results section: "Our ability to link protein folding to antibiotic resistance gives us a unique opportunity to select for activity enhancing mutations in a chaperone.
Our ability to link protein folding to antibiotic resistance gives us a unique opportunity to select for activity-enhancing mutations in a chaperone.
Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.
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