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It can be speculated that shorter selection intervals than the two weeks employed here might be sufficient to obtain UTF1 selected cultures, perhaps even without stable integration of the selection cassette into the iPS genome.
As SPC is specifically expressed by ATIICs, only hESC-derived ATIICs can express NEOR and survive in G418 selected cultures.
After retesting, the selected cultures were cloned and recloned using the limiting dilution technique and the isotypes were determined.
The selected cultures were subjected to various standard biochemical and physiological methods as per the schemes outlined in the Bergey's Manual of Systematic Bacteriology Holt and Bergey (1994).
Cells were transfected with shRNA to knock down ERK1 expression, and the selected cultures were analyzed for expression levels and response to IM (fig. 3F).
We screened for tumor-reactive antibodies from patient B cell cultures and approximated frequency and specificity of selected cultures to tumor cells.
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According to the growth curves calculated for each selected culture, maximum biomass was reached at day 4 (Fig. 1).
Growth controls showed that C. vulgaris was the species with the greatest cell abundance after 96 h in the selected culture medium (2.4 ± 0.3 × 106 cell ml-1).
The biomass value obtained (40.3 g cell dry weight l−1) employing the selected culture conditions agreed with that predicted by the model.
A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH.
A potential improvement to current strategies is to identify a robust high-lipid producing microalgae strain which thrives in selected culture conditions that discourage invasive species (Do Nascimento et al.2012; Larkum et al.2012).2012
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