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However, specific targeting of selected cell types is not possible with CPPs, and protein transfer is relatively inefficient53, 54.
Multifunctional constructs providing a proper environment for adhesion and growth of selected cell types are needed for most tissue engineering and regenerative medicine applications.
Whole-organ scaffolds can be created by detergent perfusion via the native vasculature, generating an acellular matrix suitable for recellularization with selected cell types.
We suggest that local drug delivery to selected cell types could be facilitated by employing targeted nanoparticles designed specifically to utilize alternative membrane transport mechanisms.
Pseudotyping, using AAV serotype 2 terminal repeats to generate designer shells capable of transducing selected cell types, enables the packaging of common genomes into multiple serotypes virions to directly compare gene expression and tropism.
In reporter mice in which selected cell types express fluorescent proteins, imaging after infection with fluorescent pathogens (lymphocytic choriomeningitis virus, Trypanosoma brucei or Plasmodium berghei) has shown strong recruitment to the cortical meninges of immune cells, including neutrophils, T cells, and putative dendritic cells and macrophages.
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Thus, unlike most routes of unconventional secretion, the default tTG secretion pathway is designed to operate in a wide variety rather than in a selected cell type, as is the case for FGF2 in fibroblasts [6], [9] or IL-1β in macrophages [10]-[13] [10]-[13]
For each selected cell type, we select at most two gene expression profiles from each platform in the same experiment.
From these data we propose that a single value the Bioenergetic Health Index (BHI can be calculated to represent the patient's composite mitochondrial profile for a selected cell type.
The effector molecules are genetically encoded, which allow manipulations to be targeted to select cell types.
But recent reports of methods to select cell types of interest could provide opportunities both to compare differentiation efficiencies between patients quantitatively and to answer novel questions about human neurons and glia.
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