2 SNPs selection and genotyping SLC30A8 SNPs, rs7002176, rs1995222 and rs13266634 were been selected based on previous studies [ 18, 48].
The doses of BBR and AG490 were selected based on previous reports24,25,26,27.
The low dose was selected based on previous toxicological studies of MPs in aquatic organisms24,29.
The concentrations of baclofen and GABA were selected based on previous in vitro electrophysiological studies in lampreys43.
The dose in the present experiment was selected based on previous relevant reports27,28 and our preliminary results29.
Drug doses were selected based on previous experiments in our laboratory reported in other publications.
Inhibitors were selected on the basis of their molecular targets and initial concentrations employed were selected based on previous literature reports20,21,22,23,24,25,26,27.
In this study, we used H2O2 to mimic oxidative stress with concentrations selected based on previous studies and suitability for mimicking pathological conditions28,38,39.
The delta winglet VG design with length (s) and height (H) is selected based on previous studies.
The factors under investigation were selected based on previous experiences and also the restrictions existing in the process.
The surfactant and polymer are selected based on previous laboratory screening and evaluation (Han et al. 2013).
