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In this study, the clinical common pathogens were selected as negative controls, but no Zika virus RNA was amplified.
Fifty more samples from women carrying normal female fetuses were also selected as negative controls.
Deionized water and H2O2 (100 μM) were selected as negative and positive controls, respectively.
Three random sets of 135 TFs not expressed in MSCs were selected as negative control sets.
Donors were selected as negative for any recent infection and with no history of Leishmaniasis.
At first, the 34 675 non-piRNA ncRNA were selected as negative samples.
Similar(43)
20573, 22501, 19375, and 20723 probes were selected as negatives for CREB, E2F1, MAX, and YY1, respectively (Table 1).
Out of a total of approximately 100,000 colonies plated, 8,000 negatives were counted and then finally 3532 (3.5% of those screened) well isolated colonies were selected as negatives for further analysis.
All of the results indicated that clones selected as negatives in the NSH and examined further by northern hybridization were indeed either not expressed or expressed only to a low extent in glucose-grown fungus, but induced to considerably higher levels in one or more of the complex carbohydrates or starved fungal cultures.
A representative AFI-negative (AFI–) area (green colour) was then selected as a negative control (see online supplementary figure S1B).
The yellow fever virus was theoretically selected as the negative control.
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