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It's irreverent, jarring, not for kids, and is set to a puckishly selected array of popular songs.
The strand specificity was also evident with genes for non-coding RNAs where we selected array elements for both orientations (Table 1).
The maximum height of the hook-plot reflects the relevant 3′/5′-intensity gradient of the selected array enabling the unbiased comparison of differentially expressed genes under variable RNA quality.
The quantiles of distributions for both metrics for selected array types are available as supplementary material and can be used to evaluate the quality level of any new microarray experiment.
We selected array positions that had significant fold ratios (P <0.05) and mean log2 fold change ratios ≤ −0.25 and ≥0.25 (emphasized in the Volcano plots in Figure 1) which corresponded to actual fold changes of <0.84 and >1.19, respectively.
Selected ion electropherograms were generated with a 10 mDa window for a selected array of metabolites, and the intensities of the detected peaks were noted.
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We selected arrays and genes with greater than 75% good data (representing the amount of data passing the spot criteria).
All selected arrays were human cancer samples hybridized onto genome-wide array platforms.
We compared methods by analysing two randomly selected arrays for each of 1000 runs.
These probesets are further filtered on the basis of signal intensity range defined by the user for selected arrays.
Afterwards, we quantile normalized the 286 selected arrays by employing library limma implemented in R in order to make experiments comparable with each other.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com