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Stable transfectants, constitutively expressing EGFP-Full NS3-4A were selected and maintained in a medium containing zeocin (100µg/ml and 5µg/ml for selection and maintenance, respectively).
Ideally, these R&D projects would have been selected and maintained for their maximum ability to produce outcomes.
The stable replicon-containing cells were selected and maintained in medium containing 1 mg/mL G418 geneticin (Invitrogen, USA).
Oocytes with an extruded first polar body, a round shape and an intact cytoplasm were selected and maintained in the manipulation medium for subsequent experiments.
If there were infrastructure available, the sensor locations could be selected and maintained on regular grid, as considered, for example, in [6, 17].
Over millennia, humans have slowly selected and maintained numerous coconut varieties, used for many purposes.
This reporter strain was selected and maintained using minimal medium plates lacking histidine.
In all, 30 male animals with various maturities were selected and maintained in sea water tanks with aeration.
Therefore one particular orientation must be selected and maintained for each stack at the re-sampling step.
Cells transfected with pP series plasmids containing the pac gene were selected and maintained with 54 µg/ml puromycin.
After homologous recombination, clones were selected and maintained in the presence of hygromycin B 150 µg/mL (Invitrogen).
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