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3-Aminopropyltriethoxysilane (3-APTES) was selected to activate the entire membrane thanks to its high reactivity [26].
This study generated deep RNA-Seq data from yeast incubated under many different environmental conditions and stresses, selected to activate a broad variety of gene expression patterns and regulatory networks.
The 300 cd/m blue light condition used in this study was specifically selected to activate melanopsin preferentially over rods and cones, as melanopsin has peak sensitivity in the action spectrum of light around 480 nm and requirement of high intensity [ 1, 4, 6].
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In summary, the lack of correlation between JNK and p53 phosphorylation pattern in selected tissues suggests that the regulation of JNK signaling could play a minor role in regulation of apoptosis (mediated by p53) during torpor, and the activation of JNK in selected tissues functions to activate other downstream targets, which have yet to be identified in the lemur.
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