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The immune response, however, can be different between individual mice making it possible to select the mouse that produces antibodies with favorable properties.
Briefly, the heterozygous offspring of the N4 generation were screened with polymorphic markers on chromosome 17 to select the mouse with the shortest 129Sv-derived chromosomal segment.
Using these results, it is possible to select the mouse model that most closely represents a group of human breast cancer for the signaling pathways of interest.
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We have selected the mouse aquaporin 4 as a representative of mammalian aquaporins.
We selected the mouse as the homologous species to humans due to the wider coverage of the annotated genes.
We established transgenic mouse lines by backcrossing the founders to B6C3F1 hybrid mice, and selected the mouse line with highest expression levels of WT (tgWT) or N88S/S90L mutant (tgMT) Seipin in the central nervous system (CNS).
Because we aimed to perform the assays in the physiological context of the heart, we selected the mouse genome region syntenic to human 4q25 and analysed chromatin from the atria and ventricles of adult mouse hearts.
AK-K was involved in study design, selected the mouse homologous genes, designed and prepared the probes, performed the RNA in situ hybridisation and Northern blot hybridisation as well as the data analysis and the search for candidate genes.
To this end, we selected the mouse Rosa26 locus, which exhibits apparently tissue-ubiquitous expression and into which reporter sequences have successfully been inserted downstream of the promoter (Zambrowicz et al., 1997; Soriano, 1999; Srinivas et al., 2001).
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