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gambiae PEST sequence using primer select software (Lasergene, DNA Star).
Primers were designed using primer select software of DNA star (Madison, WI) according to the parameters outlined in the BioRad i-cycler manual.
Primers specific for the amplification of the transcripts were chosen using the 'Primer Select' software, and the specificity confirmed by carrying out a detailed BLAST search.
Primer sequences for CASP9 and DFFA were selected by means of the DNASTAR primer select software (LASERGENE, Madison, WI, USA; data available on request).
Primer pairs used for quantification were designed using Primer Select software (DNASTAR, Wisconsin, USA) from the available GENBANK and/or ENSEMBL sequences, covering different exons in order to assure the amplification of the cDNA.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com