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Enzyme-linked immunosorbent assays (ELISA) was used to determine the concentration of select mouse cytokines.
Here, we select mouse (Mus musculus) as a model vertebrate organism, since genetic techniques are well established for mice, and they have many genes orthologous to genes in humans.
Humanization of 3D6 was effected by a combination of CDR grafting of mouse residues from 3D6 VL and VH into human frameworks, and homology model guided retention of select mouse framework residues in the initial humanized version of the monoclonal antibody (mAb) [ 31, 32, 35, 44, 45].
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The method we describe here could provide a quantitative means to select mice and rats with the highest levels of coat color chimerism.
Screening NOD mice for elevated blood glucose allowed us to efficiently select mice with impaired response to glucose challenge, which is indicative of the most advanced stages of insulitis (Figure 6A) [27].
Neutropenia was confirmed in select mice on postinfection days 1, 3, and 6.
We also recorded real-time activity levels and are imaging selected mouse strains by magnetic resonance imaging and computed tomography.
The next sets of experiments were then performed in selected mouse strains.
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