In addition, the ICAM-1/P-selectin deficiency was confirmed in our laboratory in randomly selected litter mice tissue samples using RT-PCR and immunohistochemical staining of the liver tissue, as previously published by our laboratory (18).
All piglets in the selected litters were examined.
For the selected litters, the average age at weaning was 37.2 days, with standard deviation 5.6 days and range 32 49 days.
However, the loss of weanling pigs in the selected litters was small, so none of the litters ended up with less than 4 full sets.
From each of these ten herds, ten randomly selected litters in their second week of life were sampled and tested as described below (section 'Parasitological methods').
For 10 randomly selected litters per group, one male ("C") was selected to provide epididymal sperm for in utero insemination, and another male ("D") was bred to two untreated females.
Select a litter that is widely available.
Select a litter that is as dust-free as possible, since dust can irritate cats' lungs.
All-state selections littered both rosters, with Blum and Maguire selected in the ninth round of the N.H.L. draft that spring.
For the P30 animals, given that a single brain was selected per litter, the laser-excised hippocampal pyramidal tissues from the left hemisphere were pooled and used for assessing CGI methylation.
The parents of the V16 females used in this study, stored as vitrified embryos, were thawed and transferred to females from another line, also selected for litter size at weaning (line A [ 27, 28]).
