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He borrowed only select fragments of the original vocals just to remind people they're remixes, not new joints.
A naive approach would be to select fragments as above, but with minimal, rather than maximal, MI.
Magnetic beads linked to streptavidin (Dynal, Life Technologies, Grand Island, New York, USA) were used to select fragments containing microsatellites hybridized to the 5′ biotinylated probe (GA 10.
Agarose gel electrophoresis was carried out to select fragments of a suitable size that was enriched by PCR amplification to construct the cDNA library.
To select fragments containing microsatellites, ligation products were hybridized with a 5′-biotinylated repeat oligonucleotide probe (GA 12 at 60°C overnight.
For each library, about nine micrograms of DNA were digested with Sau3AI (Amersham Biosciences, UK) and electrophoresed in 0.8% low melting agarose gels to select fragments ranging from 200 to 600 bp.
Similar(51)
X-ray co-crystal structures of select fragment hits confirmed binding and suggested a path for medicinal chemistry optimization.
Thermo Scientific Pinpoint software was used to predict candidate peptides, select fragment ions, and build the MS instrument method.
The selected fragments followed the selection strategies for RNAi in wheat [ 53].
After size selection, we amplified the selected fragments using Illumina-supplied PCR primers.
Much deeper sequencing will be needed to ensure complete or sufficient coverage of the selected fragments, in comparison to unbiased selection techniques such as PCR.
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