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Module 3 uses a modified version of Sander's formula, later updated by Rost to select alignments based on both identity and alignment length.
qCount provides fine-grained control over quantification, for example to include only alignments that are (anti- sense to the query region, to select anti- sensebased on mapping qualito or thereport counts for exon–exon junctions.
Unlike other aligners that report all, or one, alignment per query, or that use simple heuristics to select alignments, YAHA uses a directed acyclic graph to find the optimal set of alignments that cover a query using a biologically relevant breakpoint penalty.
All known protein sequences from Arabidopsis were individually aligned against the sequences from the entire proteome of Arabidopsis using blastp to select alignments with an E-value cut off of 1x10-20 and which covered 80%% of the query sequence [ 60].
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The results indicate that travel-time cost, which is often neglected by highway agencies in selecting alignments, significantly affects the alignment optimization.
Selected alignments, condensed by location, were used to extract surrounding putative precursor sequences from genome.
All the selected alignments (one alignment per read) were used to annotate and quantify splicing events.
Based on sequence identity, we selected alignments of five nearly full-length genome sequences.
For the selected alignments, all unaligned bases in 3′ ends of local alignments were regarded as non-templated additions.
The selected alignments were edited and manually refined with the program ED of the MUST package [ 51].
The ten selected alignments ranged in length from 155 to 648 amino acids and from 14% to 60% missing residues.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com