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Marked tumour areas were selected with a sterile razor blade.
A sample of culture was selected using a sterile plastic loop and re-suspended in 200 μl of 5 % Chelex 100 (BioRad) in a microcentifuge tube.
White colonies on the plates were selected with a sterile toothpick, transferred to LB broth (1% tryptone, 0.5% yeast extract, and 1% NaCl, pH 7.0; 10 mg ampicillin), and incubated at 37°C for 12 14 h with shaking at about 180 rpm.
Ten millilitres of whole blood was taken from the jugular vein of each selected cow, using a sterile 10-ml syringe and 18 × 1½ in.
Other is to select male sterile plants that would be ratooned under low temperature for harvesting BC1F2 seeds.
Therefore we selected a feeding RNAi duration so that the worms could still produce embryos but were partially sterile.
On each farm the square root of the number of veal calves (a minimum of 10 and a maximum of 25 calves for each farm) was randomly selected and sampled using a sterile cotton-wool swab (Cultiplast®).
The areas with the highest neoplastic cell content (>80%) were selected and microdissected with a sterile needle under microscopic control.
Transformants (T1 generation) were selected on sterile germination medium [36] containing 75 mg/L kanamycin.
After infection, cells with integrated lentivirus were selected by sterile sorting for GFP.
At the end of the study (d 42), one representative broiler chicken per pen, close to the average body weight, was selected for blood sampling using a sterile needle and heparinized vacuum tube.
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