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13 However, although the markers used to estimate a putative QTL follow a testcross segregation type, the QTL may be segregating like an intercross gene given the parents' outcrossing nature.
Markers were re-coded using genotype codes for linkage analysis using (Kyasma, Wageningen, NL), according to their segregation type; AB × AA or AB × BB (segregating in the female 'M.27' genotype) were coded and AA × AB or BB × AB (segregating in the male 'M.116' genotype) were coded .
Segregating DArTs markers present in the two sharing parents (dominant marker segregation type) were discarded, as they were poorly informative and not numerous in our data.
It gives the segregation type for each marker.
All markers were re-coded by their segregation type according to the cross-pollinated coding scheme (CP) for analysis.
The segregation type 'ab × cd' represent that the two alleles of one marker are different in both parents.
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However, the accuracy of the genetic position assigned to each marker is limited by the number of progenies in which it is segregating, the segregation types in each progeny, the presence of markers with distorted segregations and the possible existence of differences in recombination rates among the progenitor cultivars.
Table 1 tabulates parental cross types at the QTL, and the offspring segregation types for each backcross.
Joinmap is able to combine data of several segregation types to construct a consensus genetic map.
The segregation types that were heterozygous in both parents were used as bridge markers.
However, most banding patterns from the SSCP analyses could be categorized into the five segregation types shown in Table 4.
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