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The analysis of recombination events from marker segregation data is especially helpful when a large number of markers segregate in a single mapping population.
The inheritance of resistance in G978 was studied using M4 and BC1F2 segregation data.
Segregation data for all markers are available in Table S1.
A corresponding analysis of untrimmed sequences again gave complete segregation (data not shown).
In addition, cells expressing these mutants had no obvious defect in bipolar spindle formation and chromosome segregation (data not shown).
The segregation data of the remaining markers were studied with the program MultiPoint ([95] [97]; http://www.multiqtl.com).
For 39 of them, the segregation data also enabled us to orient them on the chromosomes (Table S5).
The segregation data of the markers allowed us to map 265 sequence scaffolds of the Nasonia genome assembly 1.0 (Table 1; Table S5).
Two of the lines (AsML10 and AsML15) showed an insertion on the X chromosome, as assessed by both segregation data and in situ hybridisation analysis (data not shown).
Segregation data were scored manually.
Segregation data suggested that PEN1 is not epistatic to PSS1.
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