Sentence examples for segregation assays from inspiring English sources
The fission yeast wild-type bub1+ gene has been C-terminally tagged with GFP, such that it is expressed from its own promoter at the endogenous locus, and a range of checkpoint and chromosome segregation assays demonstrate that it is fully functional [21] [23].
The fission yeast wild-type mad3+ gene has been C-terminally tagged with GFP, such that it is expressed from its own promoter at the endogenous locus, and a range of checkpoint and chromosome segregation assays demonstrate that it is fully functional [12], [21], [22].
The 20-kDa SNAP-tag did not interfere with the function of either protein, as revealed in nuclear segregation assays.
Previously, our laboratory developed a color segregation assay in which a minisatellite was inserted into the ADE2 gene in the yeast Saccharomyces cerevisiae to monitor alteration events.
We previously developed a colony color segregation assay to monitor minisatellite repeat alterations in yeast (Kelly et al. 2007, 2011, 2012).
We previously reported a unique color segregation assay that allows us to monitor minisatellite instability occurring in S. cerevisiae cells (Kelly et al. 2007, 2011, 2012).
A segregation assay was used to identify lines harboring a single transgene copy, by culturing the T2 transgenic plants on half-strength MS medium containing 50 mg L−1 of kanamycin.
The plasmid mis-segregation assay is not a good one, the data not particularly impressive, and adds very little.
The F2 population showing monogenic segregation was regarded as the mapping population, thereby subjecting to the bulked-segregant assay (BSA) for quickly mapping chromosomal region involving the target gene.
Most of the markers mapped in this study were localized to a single map position, as was also the case for the majority of markers mapped with the wheat 90K array (Wang et al. 2014), indicating that most SNP assays are specific to a single locus and/or assaying segregation of a single locus.
CWT and LL completed the tissue culture experiments, enzyme activity assays, and pgd3-umu1 co-segregation analysis.
