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To prevent this factor from contributing to estimates of mutational (among-line) variance, a fixed effect was fitted in all analyses to remove the mean difference in trait expression between the two groups of lines with the two alternative forms of this segregating factor.
We observed a segregating factor in the MA lines that must have been present in the ancestor and was therefore not a product of mutation during the experiment (Supporting Information, File S1).
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These studies suggest that sex determination in some amphibians may depend upon more than a single, segregating genetic factor.
Compartmentalization similarly regulates molecular interactions by segregating the factors, which is exemplified by steroid hormone receptors that are located outside of the nucleus in their inactive state, yet contact with the ligand leads to nuclear translocation and DNA binding [20], [38].
Overall, our results validate D. melanogaster as a model for uncovering the genetic basis of chemotoxicity and suggest the genetic basis of MTX toxicity is due to a handful of genes each harboring multiple segregating regulatory factors.
Conversely, for many quantitative characters a small number of loci have been found to have large effects, so may be studied as segregating Mendelian factors, while other loci affecting the same character have lesser effects, so are not amenable to Mendelian analysis.
However, few QTL resolved in previous studies directly addressed photoperiod sensitivity and photoperiod sensitivity was not considered to be a major factor segregating in most cross.
These indicate that a key factor segregating the pattern of miRNA expression in these human cell subsets is the maturation stage of the individuals from which the cells were isolated.
In work by Garnero et al [31], 10 markers segregated into 5 factors: Factor 1 comprised markers of bone (S-PINP, U-CTX-I) and cartilage (U-CTX2) turnover; factor 2 comprised of SCOMP, S-HA, and S-PIIINP; factor 3 comprised of markers of systemic inflammation S-CRP and S-YKL-40; and factors 4 and 5 comprised of MMP-1 and MMP-3 respectively.
The seven most informative motifs from MEME analysis proved useful in segregating functional sigma factors from sequences that bore superficial similarity to sigma factors (false positives), and allowed us to differentiate PA sigma factors from ECF sigma factors (Tables 2 and 3, also see Additional file 3 for the complete MEME results).
Principal component analysis (PCA) and factor analysis segregated anthropogenic contamination as a premier factor in influencing concentrations, followed by a second lithogenic factor.
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